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Literature summary for 2.7.3.3 extracted from
- Characterization of four arginine kinases in the ciliate Paramecium tetraurelia Investigation on the substrate inhibition mechanism (2017), Int. J. Biol. Macromol., 101, 653-659 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) | Paramecium tetraurelia |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D77A | arginine kinase AK3, Km-value for L-arginine is lower than wild-type value. Ki-value for L-arginine is 1.9 fold higher than the wild-type value | Paramecium tetraurelia |
| D78A | arginine kinase AK3, Km-value for L-arginine is higher than wild-type value. Ki-value for L-arginine is 1.14fold higher than the wild-type value | Paramecium tetraurelia |
| G298R | arginine kinase AK3, enzymatic activity is significantly reduced. Ki-value for L-arginine is 1.8fold higher than the wild-type value | Paramecium tetraurelia |
| Q80A | arginine kinase AK3, Km-value for L-arginine is lower than wild-type value. Ki-value for L-arginine is 1.5fold higher than the wild-type value | Paramecium tetraurelia |
| Q80S | arginine kinase AK3, Km-value for L-arginine is lower than wild-type value. Ki-value for L-arginine is 2.2fold higher than the wild-type value | Paramecium tetraurelia |
| R298G | arginine kinase AK4, enzymatic activity of the mutant arginine kinase AK4 is comparable with that of the wild-type arginine kinase AK3. Ki-value for L-arginine is 3.8fold higher than the wild-type value | Paramecium tetraurelia |
| S79A | arginine kinase AK3, loss of substrate inhibition by L-arginine. Ki-value for L-arginine is 8.3fold higher than the wild-type value | Paramecium tetraurelia |
| V81A | arginine kinase AK3, Km-value for L-arginine is higher than wild-type value. Ki-value for L-arginine is 12.2 fold higher than the wild-type value | Paramecium tetraurelia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-arginine | arginine kinase AK3 shows substrate inhibition. Residue S79 is essential for this phenomenon | Paramecium tetraurelia |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme Q80S | Paramecium tetraurelia | |
| 0.035 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme Q80A | Paramecium tetraurelia | |
| 0.065 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme D77A | Paramecium tetraurelia | |
| 0.076 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, wild-type enzyme | Paramecium tetraurelia | |
| 0.124 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme G298R | Paramecium tetraurelia | |
| 0.284 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK4, mutant enzyme R298G | Paramecium tetraurelia | |
| 0.348 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme S79A | Paramecium tetraurelia | |
| 0.386 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK4, wild-type enzyme | Paramecium tetraurelia | |
| 0.546 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme D78A | Paramecium tetraurelia | |
| 0.558 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme V81A | Paramecium tetraurelia | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paramecium tetraurelia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paramecium tetraurelia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-arginine | enzymatic activity of arginine kinase AK4 is less by 3% than that of arginine kinase AK3 | Paramecium tetraurelia | ADP + Nomega-phospho-L-arginine | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AK1 | - |
Paramecium tetraurelia |
| AK2 | - |
Paramecium tetraurelia |
| AK3 | - |
Paramecium tetraurelia |
| AK4 | - |
Paramecium tetraurelia |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.36 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, wild-type enzyme | Paramecium tetraurelia | |
| 4.96 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme D78A | Paramecium tetraurelia | |
| 6.67 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme Q80A | Paramecium tetraurelia | |
| 7.66 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme V81A | Paramecium tetraurelia | |
| 8.11 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme D77A | Paramecium tetraurelia | |
| 9.57 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme Q80S | Paramecium tetraurelia | |
| 16.5 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK4, mutant enzyme R298G | Paramecium tetraurelia | |
| 36.2 | - |
L-arginine | 25°C, pH not specified in the publication, arginine kinase AK3, mutant enzyme S79A | Paramecium tetraurelia | |
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